Design and evaluation of genome-wide libraries for RNA interference screens

RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for...

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Detalles Bibliográficos
Autores principales: Horn, Thomas, Sandmann, Thomas, Boutros, Michael
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911109/
https://www.ncbi.nlm.nih.gov/pubmed/20550664
http://dx.doi.org/10.1186/gb-2010-11-6-r61
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author Horn, Thomas
Sandmann, Thomas
Boutros, Michael
author_facet Horn, Thomas
Sandmann, Thomas
Boutros, Michael
author_sort Horn, Thomas
collection PubMed
description RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/.
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spelling pubmed-29111092010-07-28 Design and evaluation of genome-wide libraries for RNA interference screens Horn, Thomas Sandmann, Thomas Boutros, Michael Genome Biol Software RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/. BioMed Central 2010 2010-06-15 /pmc/articles/PMC2911109/ /pubmed/20550664 http://dx.doi.org/10.1186/gb-2010-11-6-r61 Text en Copyright ©2010 Horn et al.; licensee BioMed Central Ltd http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Software
Horn, Thomas
Sandmann, Thomas
Boutros, Michael
Design and evaluation of genome-wide libraries for RNA interference screens
title Design and evaluation of genome-wide libraries for RNA interference screens
title_full Design and evaluation of genome-wide libraries for RNA interference screens
title_fullStr Design and evaluation of genome-wide libraries for RNA interference screens
title_full_unstemmed Design and evaluation of genome-wide libraries for RNA interference screens
title_short Design and evaluation of genome-wide libraries for RNA interference screens
title_sort design and evaluation of genome-wide libraries for rna interference screens
topic Software
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911109/
https://www.ncbi.nlm.nih.gov/pubmed/20550664
http://dx.doi.org/10.1186/gb-2010-11-6-r61
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AT boutrosmichael designandevaluationofgenomewidelibrariesforrnainterferencescreens

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