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Temporal resolution of protein–protein interactions in the live-cell plasma membrane

We have recently devised a method to quantify interactions between a membrane protein (“bait”) and a fluorophore-labeled protein (“prey”) directly in the live-cell plasma membrane (Schwarzenbacher et al. Nature Methods 5:1053–1060 2008). The idea is to seed cells on surfaces containing micro-pattern...

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Detalles Bibliográficos
Autores principales: Weghuber, Julian, Sunzenauer, Stefan, Plochberger, Birgit, Brameshuber, Mario, Haselgrübler, Thomas, Schütz, Gerhard J.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911529/
https://www.ncbi.nlm.nih.gov/pubmed/20574782
http://dx.doi.org/10.1007/s00216-010-3854-x