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Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation

BACKGROUND: The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells...

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Detalles Bibliográficos
Autores principales: Faize, Mohamed, Faize, Lydia, Burgos, Lorenzo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912785/
https://www.ncbi.nlm.nih.gov/pubmed/20637070
http://dx.doi.org/10.1186/1472-6750-10-53