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Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation
BACKGROUND: The routine generation of transgenic plants involves analysis of transgene integration into the host genome by means of Southern blotting. However, this technique cannot distinguish between uniformly transformed tissues and the presence of a mixture of transgenic and non-transgenic cells...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912785/ https://www.ncbi.nlm.nih.gov/pubmed/20637070 http://dx.doi.org/10.1186/1472-6750-10-53 |