Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena

BACKGROUND: Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding. RESULTS: In this stud...

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Detalles Bibliográficos
Autores principales: Busch, Clara Jana-Lui, Vogt, Alexander, Mochizuki, Kazufumi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912859/
https://www.ncbi.nlm.nih.gov/pubmed/20626890
http://dx.doi.org/10.1186/1471-2180-10-191

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912859/
https://www.ncbi.nlm.nih.gov/pubmed/20626890
http://dx.doi.org/10.1186/1471-2180-10-191

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