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Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena
BACKGROUND: Epitope tagging is a powerful strategy to study the function of proteins. Although tools for C-terminal protein tagging in the ciliated protozoan Tetrahymena thermophila have been developed, N-terminal protein tagging in this organism is still technically demanding. RESULTS: In this stud...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912859/ https://www.ncbi.nlm.nih.gov/pubmed/20626890 http://dx.doi.org/10.1186/1471-2180-10-191 |