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Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell
Characterizing the capabilities, key dependencies, and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful reaction knockouts. The integration of combinatorial methods...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914339/ https://www.ncbi.nlm.nih.gov/pubmed/20706609 http://dx.doi.org/10.1155/2010/415148 |
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author | De Martino, A. Granata, D. Marinari, E. Martelli, C. Van Kerrebroeck, V. |
author_facet | De Martino, A. Granata, D. Marinari, E. Martelli, C. Van Kerrebroeck, V. |
author_sort | De Martino, A. |
collection | PubMed |
description | Characterizing the capabilities, key dependencies, and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful reaction knockouts. The integration of combinatorial methods with sampling techniques to explore the space of viable flux states may provide crucial insights on this issue. We assess the replaceability of every metabolic conversion in the human red blood cell by enumerating the alternative paths from substrate to product, obtaining a complete map of he potential damage of single enzymopathies. Sampling the space of optimal steady state fluxes in the healthy and in the mutated cell reveals both correlations and complementarity between topologic and dynamical aspects. |
format | Text |
id | pubmed-2914339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-29143392010-08-12 Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell De Martino, A. Granata, D. Marinari, E. Martelli, C. Van Kerrebroeck, V. J Biomed Biotechnol Research Article Characterizing the capabilities, key dependencies, and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful reaction knockouts. The integration of combinatorial methods with sampling techniques to explore the space of viable flux states may provide crucial insights on this issue. We assess the replaceability of every metabolic conversion in the human red blood cell by enumerating the alternative paths from substrate to product, obtaining a complete map of he potential damage of single enzymopathies. Sampling the space of optimal steady state fluxes in the healthy and in the mutated cell reveals both correlations and complementarity between topologic and dynamical aspects. Hindawi Publishing Corporation 2010 2010-06-30 /pmc/articles/PMC2914339/ /pubmed/20706609 http://dx.doi.org/10.1155/2010/415148 Text en Copyright © 2010 A. De Martino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article De Martino, A. Granata, D. Marinari, E. Martelli, C. Van Kerrebroeck, V. Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell |
title | Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell |
title_full | Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell |
title_fullStr | Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell |
title_full_unstemmed | Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell |
title_short | Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell |
title_sort | optimal fluxes, reaction replaceability, and response to enzymopathies in the human red blood cell |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914339/ https://www.ncbi.nlm.nih.gov/pubmed/20706609 http://dx.doi.org/10.1155/2010/415148 |
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