Cargando…

Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell

Characterizing the capabilities, key dependencies, and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful reaction knockouts. The integration of combinatorial methods...

Descripción completa

Detalles Bibliográficos
Autores principales: De Martino, A., Granata, D., Marinari, E., Martelli, C., Van Kerrebroeck, V.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914339/
https://www.ncbi.nlm.nih.gov/pubmed/20706609
http://dx.doi.org/10.1155/2010/415148
_version_ 1782184754633768960
author De Martino, A.
Granata, D.
Marinari, E.
Martelli, C.
Van Kerrebroeck, V.
author_facet De Martino, A.
Granata, D.
Marinari, E.
Martelli, C.
Van Kerrebroeck, V.
author_sort De Martino, A.
collection PubMed
description Characterizing the capabilities, key dependencies, and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful reaction knockouts. The integration of combinatorial methods with sampling techniques to explore the space of viable flux states may provide crucial insights on this issue. We assess the replaceability of every metabolic conversion in the human red blood cell by enumerating the alternative paths from substrate to product, obtaining a complete map of he potential damage of single enzymopathies. Sampling the space of optimal steady state fluxes in the healthy and in the mutated cell reveals both correlations and complementarity between topologic and dynamical aspects.
format Text
id pubmed-2914339
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-29143392010-08-12 Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell De Martino, A. Granata, D. Marinari, E. Martelli, C. Van Kerrebroeck, V. J Biomed Biotechnol Research Article Characterizing the capabilities, key dependencies, and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful reaction knockouts. The integration of combinatorial methods with sampling techniques to explore the space of viable flux states may provide crucial insights on this issue. We assess the replaceability of every metabolic conversion in the human red blood cell by enumerating the alternative paths from substrate to product, obtaining a complete map of he potential damage of single enzymopathies. Sampling the space of optimal steady state fluxes in the healthy and in the mutated cell reveals both correlations and complementarity between topologic and dynamical aspects. Hindawi Publishing Corporation 2010 2010-06-30 /pmc/articles/PMC2914339/ /pubmed/20706609 http://dx.doi.org/10.1155/2010/415148 Text en Copyright © 2010 A. De Martino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
De Martino, A.
Granata, D.
Marinari, E.
Martelli, C.
Van Kerrebroeck, V.
Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell
title Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell
title_full Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell
title_fullStr Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell
title_full_unstemmed Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell
title_short Optimal Fluxes, Reaction Replaceability, and Response to Enzymopathies in the Human Red Blood Cell
title_sort optimal fluxes, reaction replaceability, and response to enzymopathies in the human red blood cell
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914339/
https://www.ncbi.nlm.nih.gov/pubmed/20706609
http://dx.doi.org/10.1155/2010/415148
work_keys_str_mv AT demartinoa optimalfluxesreactionreplaceabilityandresponsetoenzymopathiesinthehumanredbloodcell
AT granatad optimalfluxesreactionreplaceabilityandresponsetoenzymopathiesinthehumanredbloodcell
AT marinarie optimalfluxesreactionreplaceabilityandresponsetoenzymopathiesinthehumanredbloodcell
AT martellic optimalfluxesreactionreplaceabilityandresponsetoenzymopathiesinthehumanredbloodcell
AT vankerrebroeckv optimalfluxesreactionreplaceabilityandresponsetoenzymopathiesinthehumanredbloodcell