Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase

Peptide bond synthesis was performed on PEGA beads under microwave radiations. Classical chemical coupling as well as thermolysin catalyzed synthesis was studied, and the effect of microwave radiations on reaction kinetics, beads' integrity, and enzyme activity was assessed. Results demonstrate...

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Detalles Bibliográficos
Autores principales: Basso, Alessandra, Sinigoi, Loris, Gardossi, Lucia, Flitsch, Sabine
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915653/
https://www.ncbi.nlm.nih.gov/pubmed/20721296
http://dx.doi.org/10.1155/2009/362482
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author Basso, Alessandra
Sinigoi, Loris
Gardossi, Lucia
Flitsch, Sabine
author_facet Basso, Alessandra
Sinigoi, Loris
Gardossi, Lucia
Flitsch, Sabine
author_sort Basso, Alessandra
collection PubMed
description Peptide bond synthesis was performed on PEGA beads under microwave radiations. Classical chemical coupling as well as thermolysin catalyzed synthesis was studied, and the effect of microwave radiations on reaction kinetics, beads' integrity, and enzyme activity was assessed. Results demonstrate that microwave radiations can be profitably exploited to improve reaction kinetics in solid phase peptide synthesis when both chemical and biocatalytic strategies are used.
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spelling pubmed-29156532010-08-18 Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase Basso, Alessandra Sinigoi, Loris Gardossi, Lucia Flitsch, Sabine Int J Pept Research Article Peptide bond synthesis was performed on PEGA beads under microwave radiations. Classical chemical coupling as well as thermolysin catalyzed synthesis was studied, and the effect of microwave radiations on reaction kinetics, beads' integrity, and enzyme activity was assessed. Results demonstrate that microwave radiations can be profitably exploited to improve reaction kinetics in solid phase peptide synthesis when both chemical and biocatalytic strategies are used. Hindawi Publishing Corporation 2009 2009-03-25 /pmc/articles/PMC2915653/ /pubmed/20721296 http://dx.doi.org/10.1155/2009/362482 Text en Copyright © 2009 Alessandra Basso et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Basso, Alessandra
Sinigoi, Loris
Gardossi, Lucia
Flitsch, Sabine
Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase
title Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase
title_full Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase
title_fullStr Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase
title_full_unstemmed Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase
title_short Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase
title_sort effect of microwave radiation on enzymatic and chemical peptide bond synthesis on solid phase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915653/
https://www.ncbi.nlm.nih.gov/pubmed/20721296
http://dx.doi.org/10.1155/2009/362482
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AT sinigoiloris effectofmicrowaveradiationonenzymaticandchemicalpeptidebondsynthesisonsolidphase
AT gardossilucia effectofmicrowaveradiationonenzymaticandchemicalpeptidebondsynthesisonsolidphase
AT flitschsabine effectofmicrowaveradiationonenzymaticandchemicalpeptidebondsynthesisonsolidphase

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