Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers

The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis,...

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Detalles Bibliográficos
Autores principales: Ferreira, Rubén, Aviñó, Anna, Pérez-Tomás, Ricardo, Gargallo, Raimundo, Eritja, Ramon
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915818/
https://www.ncbi.nlm.nih.gov/pubmed/20725626
http://dx.doi.org/10.4061/2010/489060
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author Ferreira, Rubén
Aviñó, Anna
Pérez-Tomás, Ricardo
Gargallo, Raimundo
Eritja, Ramon
author_facet Ferreira, Rubén
Aviñó, Anna
Pérez-Tomás, Ricardo
Gargallo, Raimundo
Eritja, Ramon
author_sort Ferreira, Rubén
collection PubMed
description The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes. The fluorescence spectra of acridine oligomers were affected strongly upon binding to DNA. These spectral changes were used to calculate the binding constants (K). Log K were found to be in the order of 4–6.
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spelling pubmed-29158182010-08-19 Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers Ferreira, Rubén Aviñó, Anna Pérez-Tomás, Ricardo Gargallo, Raimundo Eritja, Ramon J Nucleic Acids Research Article The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes. The fluorescence spectra of acridine oligomers were affected strongly upon binding to DNA. These spectral changes were used to calculate the binding constants (K). Log K were found to be in the order of 4–6. SAGE-Hindawi Access to Research 2010-06-13 /pmc/articles/PMC2915818/ /pubmed/20725626 http://dx.doi.org/10.4061/2010/489060 Text en Copyright © 2010 Rubén Ferreira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ferreira, Rubén
Aviñó, Anna
Pérez-Tomás, Ricardo
Gargallo, Raimundo
Eritja, Ramon
Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers
title Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers
title_full Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers
title_fullStr Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers
title_full_unstemmed Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers
title_short Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers
title_sort synthesis and g-quadruplex-binding properties of defined acridine oligomers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915818/
https://www.ncbi.nlm.nih.gov/pubmed/20725626
http://dx.doi.org/10.4061/2010/489060
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