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Tumor masses support naive T cell infiltration, activation, and differentiation into effectors
Studies of T cell responses to tumors have focused on the draining lymph node (LN) as the site of activation. We examined the tumor mass as a potential site of activation after adoptive transfer of naive tumor-specific CD8 T cells. Activated CD8 T cells were present in tumors within 24 h of adoptive...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916130/ https://www.ncbi.nlm.nih.gov/pubmed/20660615 http://dx.doi.org/10.1084/jem.20092454 |
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author | Thompson, Elizabeth D. Enriquez, Hilda L. Fu, Yang-Xin Engelhard, Victor H. |
author_facet | Thompson, Elizabeth D. Enriquez, Hilda L. Fu, Yang-Xin Engelhard, Victor H. |
author_sort | Thompson, Elizabeth D. |
collection | PubMed |
description | Studies of T cell responses to tumors have focused on the draining lymph node (LN) as the site of activation. We examined the tumor mass as a potential site of activation after adoptive transfer of naive tumor-specific CD8 T cells. Activated CD8 T cells were present in tumors within 24 h of adoptive transfer and proliferation of these cells was also evident 4–5 d later in mice treated with FTY720 to prevent infiltration of cells activated in LNs. To confirm that activation of these T cells occurred in the tumor and not the tumor-draining LNs, we used mice lacking LNs. Activated and proliferating tumor-infiltrating lymphocytes were evident in these mice 24 h and 4 d after naive cell transfer. T cells activated within tumors acquired effector function that was evident both ex vivo and in vivo. Both cross-presenting antigen presenting cells within the tumor and tumor cells directly presenting antigen activated these functional CD8 effectors. We conclude that tumors support the infiltration, activation, and effector differentiation of naive CD8 T cells, despite the presence of immunosuppressive mechanisms. Thus, targeting of T cell activation to tumors may present a tool in the development of cancer immunotherapy. |
format | Text |
id | pubmed-2916130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-29161302011-02-02 Tumor masses support naive T cell infiltration, activation, and differentiation into effectors Thompson, Elizabeth D. Enriquez, Hilda L. Fu, Yang-Xin Engelhard, Victor H. J Exp Med Article Studies of T cell responses to tumors have focused on the draining lymph node (LN) as the site of activation. We examined the tumor mass as a potential site of activation after adoptive transfer of naive tumor-specific CD8 T cells. Activated CD8 T cells were present in tumors within 24 h of adoptive transfer and proliferation of these cells was also evident 4–5 d later in mice treated with FTY720 to prevent infiltration of cells activated in LNs. To confirm that activation of these T cells occurred in the tumor and not the tumor-draining LNs, we used mice lacking LNs. Activated and proliferating tumor-infiltrating lymphocytes were evident in these mice 24 h and 4 d after naive cell transfer. T cells activated within tumors acquired effector function that was evident both ex vivo and in vivo. Both cross-presenting antigen presenting cells within the tumor and tumor cells directly presenting antigen activated these functional CD8 effectors. We conclude that tumors support the infiltration, activation, and effector differentiation of naive CD8 T cells, despite the presence of immunosuppressive mechanisms. Thus, targeting of T cell activation to tumors may present a tool in the development of cancer immunotherapy. The Rockefeller University Press 2010-08-02 /pmc/articles/PMC2916130/ /pubmed/20660615 http://dx.doi.org/10.1084/jem.20092454 Text en © 2010 Thompson et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Article Thompson, Elizabeth D. Enriquez, Hilda L. Fu, Yang-Xin Engelhard, Victor H. Tumor masses support naive T cell infiltration, activation, and differentiation into effectors |
title | Tumor masses support naive T cell infiltration, activation, and differentiation into effectors |
title_full | Tumor masses support naive T cell infiltration, activation, and differentiation into effectors |
title_fullStr | Tumor masses support naive T cell infiltration, activation, and differentiation into effectors |
title_full_unstemmed | Tumor masses support naive T cell infiltration, activation, and differentiation into effectors |
title_short | Tumor masses support naive T cell infiltration, activation, and differentiation into effectors |
title_sort | tumor masses support naive t cell infiltration, activation, and differentiation into effectors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916130/ https://www.ncbi.nlm.nih.gov/pubmed/20660615 http://dx.doi.org/10.1084/jem.20092454 |
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