Use of Activity-Based Probes to Develop High Throughput Screening Assays That Can Be Performed in Complex Cell Extracts

BACKGROUND: High throughput screening (HTS) is one of the primary tools used to identify novel enzyme inhibitors. However, its applicability is generally restricted to targets that can either be expressed recombinantly or purified in large quantities. METHODOLOGY AND PRINCIPAL FINDINGS: Here, we des...

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Detalles Bibliográficos
Autores principales: Deu, Edgar, Yang, Zhimou, Wang, Flora, Klemba, Michael, Bogyo, Matthew
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916841/
https://www.ncbi.nlm.nih.gov/pubmed/20700487
http://dx.doi.org/10.1371/journal.pone.0011985

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916841/
https://www.ncbi.nlm.nih.gov/pubmed/20700487
http://dx.doi.org/10.1371/journal.pone.0011985

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