Northern blot detection of endogenous small RNAs (∼14 nt) in bacterial total RNA extracts

Here we describe a northern blot procedure that allows the detection of endogenous RNAs as small as ∼14 nt in total RNA extracts from bacteria. RNAs that small and as part of total bacterial RNA extracts usually escape detection by northern blotting. The approach combines LNA probes 5′-digoxigenin-e...

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Detalles Bibliográficos
Autores principales: Beckmann, Benedikt M., Grünweller, Arnold, Weber, Michael H. W., Hartmann, Roland K.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2919735/
https://www.ncbi.nlm.nih.gov/pubmed/20504856
http://dx.doi.org/10.1093/nar/gkq437

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2919735/
https://www.ncbi.nlm.nih.gov/pubmed/20504856
http://dx.doi.org/10.1093/nar/gkq437

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