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Northern blot detection of endogenous small RNAs (∼14 nt) in bacterial total RNA extracts
Here we describe a northern blot procedure that allows the detection of endogenous RNAs as small as ∼14 nt in total RNA extracts from bacteria. RNAs that small and as part of total bacterial RNA extracts usually escape detection by northern blotting. The approach combines LNA probes 5′-digoxigenin-e...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2919735/ https://www.ncbi.nlm.nih.gov/pubmed/20504856 http://dx.doi.org/10.1093/nar/gkq437 |