Enhancement of gene targeting in human cells by intranuclear permeation of the Saccharomyces cerevisiae Rad52 protein

The introduction of exogenous DNA in human somatic cells results in a frequency of random integration at least 100-fold higher than gene targeting (GT), posing a seemingly insurmountable limitation for gene therapy applications. We previously reported that, in human cells, the stable over-expression...

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Detalles Bibliográficos
Autores principales: Kalvala, Arjun, Rainaldi, Giuseppe, Di Primio, Cristina, Liverani, Vania, Falaschi, Arturo, Galli, Alvaro
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2919737/
https://www.ncbi.nlm.nih.gov/pubmed/20519199
http://dx.doi.org/10.1093/nar/gkq486

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2919737/
https://www.ncbi.nlm.nih.gov/pubmed/20519199
http://dx.doi.org/10.1093/nar/gkq486

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