Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation

GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure...

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Autores principales: Jinek, Martin, Fabian, Marc R., Coyle, Scott M., Sonenberg, Nahum, Doudna, Jennifer A.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920127/
https://www.ncbi.nlm.nih.gov/pubmed/20098421
http://dx.doi.org/10.1038/nsmb.1768
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author Jinek, Martin
Fabian, Marc R.
Coyle, Scott M.
Sonenberg, Nahum
Doudna, Jennifer A.
author_facet Jinek, Martin
Fabian, Marc R.
Coyle, Scott M.
Sonenberg, Nahum
Doudna, Jennifer A.
author_sort Jinek, Martin
collection PubMed
description GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.
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spelling pubmed-29201272010-08-11 Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation Jinek, Martin Fabian, Marc R. Coyle, Scott M. Sonenberg, Nahum Doudna, Jennifer A. Nat Struct Mol Biol Article GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing. 2010-01-24 2010-02 /pmc/articles/PMC2920127/ /pubmed/20098421 http://dx.doi.org/10.1038/nsmb.1768 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Jinek, Martin
Fabian, Marc R.
Coyle, Scott M.
Sonenberg, Nahum
Doudna, Jennifer A.
Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation
title Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation
title_full Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation
title_fullStr Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation
title_full_unstemmed Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation
title_short Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation
title_sort structural insights into the human gw182-pabc interaction in microrna-mediated deadenylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920127/
https://www.ncbi.nlm.nih.gov/pubmed/20098421
http://dx.doi.org/10.1038/nsmb.1768
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