Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection

BACKGROUND: Molecular DNA cloning is crucial to many experiments and with the trend to higher throughput of modern approaches automated techniques are urgently required. We have established an automated, fast and flexible low-cost expression cloning approach requiring only vector and insert amplific...

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Detalles Bibliográficos
Autores principales: Olieric, Natacha, Kuchen, Melanie, Wagen, Sandro, Sauter, Marion, Crone, Stephanie, Edmondson, Sonia, Frey, Daniel, Ostermeier, Christian, Steinmetz, Michel O, Jaussi, Rolf
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924254/
https://www.ncbi.nlm.nih.gov/pubmed/20691119
http://dx.doi.org/10.1186/1472-6750-10-56

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924254/
https://www.ncbi.nlm.nih.gov/pubmed/20691119
http://dx.doi.org/10.1186/1472-6750-10-56

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