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Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection
BACKGROUND: Molecular DNA cloning is crucial to many experiments and with the trend to higher throughput of modern approaches automated techniques are urgently required. We have established an automated, fast and flexible low-cost expression cloning approach requiring only vector and insert amplific...
Autores principales: | Olieric, Natacha, Kuchen, Melanie, Wagen, Sandro, Sauter, Marion, Crone, Stephanie, Edmondson, Sonia, Frey, Daniel, Ostermeier, Christian, Steinmetz, Michel O, Jaussi, Rolf |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924254/ https://www.ncbi.nlm.nih.gov/pubmed/20691119 http://dx.doi.org/10.1186/1472-6750-10-56 |
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