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Single-Molecule Three-Color FRET with Both Negligible Spectral Overlap and Long Observation Time
Full understanding of complex biological interactions frequently requires multi-color detection capability in doing single-molecule fluorescence resonance energy transfer (FRET) experiments. Existing single-molecule three-color FRET techniques, however, suffer from severe photobleaching of Alexa 488...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924373/ https://www.ncbi.nlm.nih.gov/pubmed/20808851 http://dx.doi.org/10.1371/journal.pone.0012270 |