Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions

BACKGROUND: The lambda Red recombination system has been used to inactivate chromosomal genes in various bacteria and fungi. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment containing antibiotic cassette flanked by homology regions to the target locus into a stra...

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Detalles Bibliográficos
Autores principales: Liang, Rubing, Liu, Jianhua
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924854/
https://www.ncbi.nlm.nih.gov/pubmed/20682065
http://dx.doi.org/10.1186/1471-2180-10-209

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924854/
https://www.ncbi.nlm.nih.gov/pubmed/20682065
http://dx.doi.org/10.1186/1471-2180-10-209

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