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Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions
BACKGROUND: The lambda Red recombination system has been used to inactivate chromosomal genes in various bacteria and fungi. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment containing antibiotic cassette flanked by homology regions to the target locus into a stra...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924854/ https://www.ncbi.nlm.nih.gov/pubmed/20682065 http://dx.doi.org/10.1186/1471-2180-10-209 |