Sequence-specific recognition of methylated DNA by human zinc-finger proteins

DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achi...

Descripción completa

Detalles Bibliográficos
Autores principales: Sasai, Nobuhiro, Nakao, Mitsuyoshi, Defossez, Pierre-Antoine
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Gene Regulation, Chromatin and Epigenetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926618/
https://www.ncbi.nlm.nih.gov/pubmed/20403812
http://dx.doi.org/10.1093/nar/gkq280
_version_ 1782185713410768896
author Sasai, Nobuhiro
Nakao, Mitsuyoshi
Defossez, Pierre-Antoine
author_facet Sasai, Nobuhiro
Nakao, Mitsuyoshi
Defossez, Pierre-Antoine
author_sort Sasai, Nobuhiro
collection PubMed
description DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achieved is largely unclear. Here, we report that the human zinc-finger proteins Kaiso, ZBTB4 and ZBTB38 can bind methylated DNA in a sequence-specific manner, and that they may use a mode of binding common to other zinc-finger proteins. This suggests that many other sequence-specific methyl binding proteins may exist.
format Text
id pubmed-2926618
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-29266182010-08-30 Sequence-specific recognition of methylated DNA by human zinc-finger proteins Sasai, Nobuhiro Nakao, Mitsuyoshi Defossez, Pierre-Antoine Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics DNA methylation is an essential epigenetic mark. Three classes of mammalian proteins recognize methylated DNA: MBD proteins, SRA proteins and the zinc-finger proteins Kaiso, ZBTB4 and ZBTB38. The last three proteins can bind either methylated DNA or unmethylated consensus sequences; how this is achieved is largely unclear. Here, we report that the human zinc-finger proteins Kaiso, ZBTB4 and ZBTB38 can bind methylated DNA in a sequence-specific manner, and that they may use a mode of binding common to other zinc-finger proteins. This suggests that many other sequence-specific methyl binding proteins may exist. Oxford University Press 2010-08 2010-04-19 /pmc/articles/PMC2926618/ /pubmed/20403812 http://dx.doi.org/10.1093/nar/gkq280 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Sasai, Nobuhiro
Nakao, Mitsuyoshi
Defossez, Pierre-Antoine
Sequence-specific recognition of methylated DNA by human zinc-finger proteins
title Sequence-specific recognition of methylated DNA by human zinc-finger proteins
title_full Sequence-specific recognition of methylated DNA by human zinc-finger proteins
title_fullStr Sequence-specific recognition of methylated DNA by human zinc-finger proteins
title_full_unstemmed Sequence-specific recognition of methylated DNA by human zinc-finger proteins
title_short Sequence-specific recognition of methylated DNA by human zinc-finger proteins
title_sort sequence-specific recognition of methylated dna by human zinc-finger proteins
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926618/
https://www.ncbi.nlm.nih.gov/pubmed/20403812
http://dx.doi.org/10.1093/nar/gkq280
work_keys_str_mv AT sasainobuhiro sequencespecificrecognitionofmethylateddnabyhumanzincfingerproteins
AT nakaomitsuyoshi sequencespecificrecognitionofmethylateddnabyhumanzincfingerproteins
AT defossezpierreantoine sequencespecificrecognitionofmethylateddnabyhumanzincfingerproteins

Ejemplares similares