Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology

We previously demonstrated high-frequency, targeted DNA addition mediated by the homology-directed DNA repair pathway. This method uses a zinc-finger nuclease (ZFN) to create a site-specific double-strand break (DSB) that facilitates copying of genetic information into the chromosome from an exogeno...

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Detalles Bibliográficos
Autores principales: Orlando, Salvatore J., Santiago, Yolanda, DeKelver, Russell C., Freyvert, Yevgeniy, Boydston, Elizabeth A., Moehle, Erica A., Choi, Vivian M., Gopalan, Sunita M., Lou, Jacqueline F., Li, James, Miller, Jeffrey C., Holmes, Michael C., Gregory, Philip D., Urnov, Fyodor D., Cost, Gregory J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926620/
https://www.ncbi.nlm.nih.gov/pubmed/20530528
http://dx.doi.org/10.1093/nar/gkq512

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926620/
https://www.ncbi.nlm.nih.gov/pubmed/20530528
http://dx.doi.org/10.1093/nar/gkq512

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