Knocking down gene function with an RNA aptamer expressed as part of an intron

We developed a powerful expression system to produce aptamers and other types of functional RNA in yeast to examine their effects. Utilizing the intron homing process, the aptamer-coding sequences were integrated into hundreds of rRNA genes, and the aptamers were transcribed at high levels by RNA po...

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Detalles Bibliográficos
Autores principales: Wang, Shengchun, Zhao, Xiaoching, Suran, Robert, Vogt, Volker M., Lis, John T., Shi, Hua
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926621/
https://www.ncbi.nlm.nih.gov/pubmed/20542918
http://dx.doi.org/10.1093/nar/gkq529

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2926621/
https://www.ncbi.nlm.nih.gov/pubmed/20542918
http://dx.doi.org/10.1093/nar/gkq529

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