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Altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression

Several studies have proposed that brain glutamate signaling abnormalities and glial pathology play a role in the etiology of major depressive disorder (MDD). These conclusions were primarily drawn from postmortem studies in which forebrain brain regions were examined. The locus coeruleus is the pri...

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Autores principales: Bernard, René, Kerman, Ilan A., Thompson, Robert C., Jones, Edward G., Bunney, William E., Barchas, Jack D., Schatzberg, Alan F., Myers, Richard M, Akil, Huda, Watson, Stanley J.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927798/
https://www.ncbi.nlm.nih.gov/pubmed/20386568
http://dx.doi.org/10.1038/mp.2010.44
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author Bernard, René
Kerman, Ilan A.
Thompson, Robert C.
Jones, Edward G.
Bunney, William E.
Barchas, Jack D.
Schatzberg, Alan F.
Myers, Richard M
Akil, Huda
Watson, Stanley J.
author_facet Bernard, René
Kerman, Ilan A.
Thompson, Robert C.
Jones, Edward G.
Bunney, William E.
Barchas, Jack D.
Schatzberg, Alan F.
Myers, Richard M
Akil, Huda
Watson, Stanley J.
author_sort Bernard, René
collection PubMed
description Several studies have proposed that brain glutamate signaling abnormalities and glial pathology play a role in the etiology of major depressive disorder (MDD). These conclusions were primarily drawn from postmortem studies in which forebrain brain regions were examined. The locus coeruleus is the primary source of extensive noradrenergic innervation of the forebrain and as such exerts a powerful regulatory role over cognitive and affective functions, which are dysregulated in MDD. Furthermore, altered noradrenergic neurotransmission is associated with depressive symptoms and is thought to play a role in the pathophysiology of MDD. In the present study we used laser-capture microdissection to selectively harvest locus coeruleus (LC) tissue from postmortem brains of MDD patients, patients with bipolar disorder (BPD), and from psychiatrically-normal subjects. Using microarray technology we examined global patterns of gene expression. Differential mRNA expression of select candidate genes was then interrogated using quantitative real-time PCR and in situ hybridization. Our findings reveal multiple signaling pathway alterations in the LC of MDD, but not BPD subjects. These include glutamate signaling genes SLC1A2, SLC1A3, GLUL, growth factor genes FGFR3 and TrkB, and several genes exclusively expressed in astroglia. Our data extend previous findings of altered glutamate, astroglial and growth factor functions in MDD for the first time to the brainstem. These findings indicate that such alterations: 1) are unique to MDD and distinguishable from BPD, and 2) affect multiple brain regions, suggesting a whole-brain dysregulation of such functions.
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spelling pubmed-29277982011-12-01 Altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression Bernard, René Kerman, Ilan A. Thompson, Robert C. Jones, Edward G. Bunney, William E. Barchas, Jack D. Schatzberg, Alan F. Myers, Richard M Akil, Huda Watson, Stanley J. Mol Psychiatry Article Several studies have proposed that brain glutamate signaling abnormalities and glial pathology play a role in the etiology of major depressive disorder (MDD). These conclusions were primarily drawn from postmortem studies in which forebrain brain regions were examined. The locus coeruleus is the primary source of extensive noradrenergic innervation of the forebrain and as such exerts a powerful regulatory role over cognitive and affective functions, which are dysregulated in MDD. Furthermore, altered noradrenergic neurotransmission is associated with depressive symptoms and is thought to play a role in the pathophysiology of MDD. In the present study we used laser-capture microdissection to selectively harvest locus coeruleus (LC) tissue from postmortem brains of MDD patients, patients with bipolar disorder (BPD), and from psychiatrically-normal subjects. Using microarray technology we examined global patterns of gene expression. Differential mRNA expression of select candidate genes was then interrogated using quantitative real-time PCR and in situ hybridization. Our findings reveal multiple signaling pathway alterations in the LC of MDD, but not BPD subjects. These include glutamate signaling genes SLC1A2, SLC1A3, GLUL, growth factor genes FGFR3 and TrkB, and several genes exclusively expressed in astroglia. Our data extend previous findings of altered glutamate, astroglial and growth factor functions in MDD for the first time to the brainstem. These findings indicate that such alterations: 1) are unique to MDD and distinguishable from BPD, and 2) affect multiple brain regions, suggesting a whole-brain dysregulation of such functions. 2010-04-13 2011-06 /pmc/articles/PMC2927798/ /pubmed/20386568 http://dx.doi.org/10.1038/mp.2010.44 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Bernard, René
Kerman, Ilan A.
Thompson, Robert C.
Jones, Edward G.
Bunney, William E.
Barchas, Jack D.
Schatzberg, Alan F.
Myers, Richard M
Akil, Huda
Watson, Stanley J.
Altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression
title Altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression
title_full Altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression
title_fullStr Altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression
title_full_unstemmed Altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression
title_short Altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression
title_sort altered expression of glutamate signaling, growth factor and glia genes in the locus coeruleus of patients with major depression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2927798/
https://www.ncbi.nlm.nih.gov/pubmed/20386568
http://dx.doi.org/10.1038/mp.2010.44
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