Validated HPLC-RI Method for the Determination of Lactulose and its Process Related Impurities in Syrup

A simple, swift with good sensitivity and reproducibility, HPLC-RI method has been developed for the quantification of lactulose and related compounds (fructose, galactose, epilactose and lactose) in oral suspension formulation. The analysis was carried out by using mobile phase (water and acetonitr...

Descripción completa

Detalles Bibliográficos
Autores principales: Nelofar, A., Laghari, A.H., Yasmin, A.
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929791/
https://www.ncbi.nlm.nih.gov/pubmed/20838536
http://dx.doi.org/10.4103/0250-474X.65027
Descripción
Sumario:A simple, swift with good sensitivity and reproducibility, HPLC-RI method has been developed for the quantification of lactulose and related compounds (fructose, galactose, epilactose and lactose) in oral suspension formulation. The analysis was carried out by using mobile phase (water and acetonitrile 75:25) at the flow rate of 1.0 ml/min. on isocratic HPLC-RI system. After manipulating mobile phase composition and mobile phase flow rate a good separation of five components was achieved within 15 minutes of run time. This study is beneficial to determine the active ingredient as well as related compounds simultaneously, without using buffer in mobile phase which causes bad resolution and has limitation to analyze on other hyphenated techniques such as LC-MS.

Ejemplares similares