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A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data
BACKGROUND: Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic range. The gold standard method for estimating DNA concentrations via qPCR is quantification cycle ([Image: see text]) standard curv...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2930010/ https://www.ncbi.nlm.nih.gov/pubmed/20814578 http://dx.doi.org/10.1371/journal.pone.0012355 |