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A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data

BACKGROUND: Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic range. The gold standard method for estimating DNA concentrations via qPCR is quantification cycle ([Image: see text]) standard curv...

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Detalles Bibliográficos
Autores principales: Boggy, Gregory J., Woolf, Peter J.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2930010/
https://www.ncbi.nlm.nih.gov/pubmed/20814578
http://dx.doi.org/10.1371/journal.pone.0012355