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Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study

BACKGROUND: Excitotoxicity is involved in the pathogenesis of a number neurodegenerative diseases, and axonopathy is an early feature in several of these disorders. In models of excitotoxicity-associated neurological disease, an excitotoxin delivered to the central nervous system (CNS), could trigge...

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Autores principales: Saggu, Sarabjit K, Chotaliya, Hiren P, Blumbergs, Peter C, Casson, Robert J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2930628/
https://www.ncbi.nlm.nih.gov/pubmed/20707883
http://dx.doi.org/10.1186/1471-2202-11-97
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author Saggu, Sarabjit K
Chotaliya, Hiren P
Blumbergs, Peter C
Casson, Robert J
author_facet Saggu, Sarabjit K
Chotaliya, Hiren P
Blumbergs, Peter C
Casson, Robert J
author_sort Saggu, Sarabjit K
collection PubMed
description BACKGROUND: Excitotoxicity is involved in the pathogenesis of a number neurodegenerative diseases, and axonopathy is an early feature in several of these disorders. In models of excitotoxicity-associated neurological disease, an excitotoxin delivered to the central nervous system (CNS), could trigger neuronal death not only in the somatodendritic region, but also in the axonal region, via oligodendrocyte N-methyl-D-aspartate (NMDA) receptors. The retina and optic nerve, as approachable regions of the brain, provide a unique anatomical substrate to investigate the "downstream" effect of isolated excitotoxic perikaryal injury on central nervous system (CNS) axons, potentially providing information about the pathogenesis of the axonopathy in clinical neurological disorders. Herein, we provide ultrastructural information about the retinal ganglion cell (RGC) somata and their axons, both unmyelinated and myelinated, after NMDA-induced retinal injury. Male Sprague-Dawley rats were killed at 0 h, 24 h, 72 h and 7 days after injecting 20 nM NMDA into the vitreous chamber of the left eye (n = 8 in each group). Saline-injected right eyes served as controls. After perfusion fixation, dissection, resin-embedding and staining, ultrathin sections of eyes and proximal (intraorbital) and distal (intracranial) optic nerve segments were evaluated by transmission electron tomography (TEM). RESULTS: TEM demonstrated features of necrosis in RGCs: mitochondrial and endoplasmic reticulum swelling, disintegration of polyribosomes, rupture of membranous organelle and formation of myelin bodies. Ultrastructural damage in the optic nerve mimicked the changes of Wallerian degeneration; early nodal/paranodal disturbances were followed by the appearance of three major morphological variants: dark degeneration, watery degeneration and demyelination. CONCLUSION: NMDA-induced excitotoxic retinal injury causes mainly necrotic RGC somal death with Wallerian-like degeneration of the optic nerve. Since axonal degeneration associated with perikaryal excitotoxic injury is an active, regulated process, it may be amenable to therapeutic intervention.
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spelling pubmed-29306282010-09-01 Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study Saggu, Sarabjit K Chotaliya, Hiren P Blumbergs, Peter C Casson, Robert J BMC Neurosci Research Article BACKGROUND: Excitotoxicity is involved in the pathogenesis of a number neurodegenerative diseases, and axonopathy is an early feature in several of these disorders. In models of excitotoxicity-associated neurological disease, an excitotoxin delivered to the central nervous system (CNS), could trigger neuronal death not only in the somatodendritic region, but also in the axonal region, via oligodendrocyte N-methyl-D-aspartate (NMDA) receptors. The retina and optic nerve, as approachable regions of the brain, provide a unique anatomical substrate to investigate the "downstream" effect of isolated excitotoxic perikaryal injury on central nervous system (CNS) axons, potentially providing information about the pathogenesis of the axonopathy in clinical neurological disorders. Herein, we provide ultrastructural information about the retinal ganglion cell (RGC) somata and their axons, both unmyelinated and myelinated, after NMDA-induced retinal injury. Male Sprague-Dawley rats were killed at 0 h, 24 h, 72 h and 7 days after injecting 20 nM NMDA into the vitreous chamber of the left eye (n = 8 in each group). Saline-injected right eyes served as controls. After perfusion fixation, dissection, resin-embedding and staining, ultrathin sections of eyes and proximal (intraorbital) and distal (intracranial) optic nerve segments were evaluated by transmission electron tomography (TEM). RESULTS: TEM demonstrated features of necrosis in RGCs: mitochondrial and endoplasmic reticulum swelling, disintegration of polyribosomes, rupture of membranous organelle and formation of myelin bodies. Ultrastructural damage in the optic nerve mimicked the changes of Wallerian degeneration; early nodal/paranodal disturbances were followed by the appearance of three major morphological variants: dark degeneration, watery degeneration and demyelination. CONCLUSION: NMDA-induced excitotoxic retinal injury causes mainly necrotic RGC somal death with Wallerian-like degeneration of the optic nerve. Since axonal degeneration associated with perikaryal excitotoxic injury is an active, regulated process, it may be amenable to therapeutic intervention. BioMed Central 2010-08-13 /pmc/articles/PMC2930628/ /pubmed/20707883 http://dx.doi.org/10.1186/1471-2202-11-97 Text en Copyright ©2010 Saggu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Saggu, Sarabjit K
Chotaliya, Hiren P
Blumbergs, Peter C
Casson, Robert J
Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study
title Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study
title_full Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study
title_fullStr Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study
title_full_unstemmed Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study
title_short Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study
title_sort wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2930628/
https://www.ncbi.nlm.nih.gov/pubmed/20707883
http://dx.doi.org/10.1186/1471-2202-11-97
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