Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins

Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 were used with MF59 adjuvant as a candidate vaccine for a phase 1 safety and immunogenicity trial. Ten of 41 vaccinee serum samples displayed a neutralization titer of ⩾1:20 against vesicular stomatitis virus (VSV)-HCV pseudotype, 15 of 36 ser...

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Autores principales: Ray, Ranjit, Meyer, Keith, Banerjee, Arup, Basu, Arnab, Coates, Stephen, Abrignani, Sergio, Houghton, Michael, Frey, Sharon E., Belshe, Robert B.
Formato: Texto
Lenguaje:English
Publicado: University Chicago Press 2010
Materias:
Major Articles and Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2931414/
https://www.ncbi.nlm.nih.gov/pubmed/20677942
http://dx.doi.org/10.1086/655902
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author Ray, Ranjit
Meyer, Keith
Banerjee, Arup
Basu, Arnab
Coates, Stephen
Abrignani, Sergio
Houghton, Michael
Frey, Sharon E.
Belshe, Robert B.
author_facet Ray, Ranjit
Meyer, Keith
Banerjee, Arup
Basu, Arnab
Coates, Stephen
Abrignani, Sergio
Houghton, Michael
Frey, Sharon E.
Belshe, Robert B.
author_sort Ray, Ranjit
collection PubMed
description Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 were used with MF59 adjuvant as a candidate vaccine for a phase 1 safety and immunogenicity trial. Ten of 41 vaccinee serum samples displayed a neutralization titer of ⩾1:20 against vesicular stomatitis virus (VSV)-HCV pseudotype, 15 of 36 serum samples tested had a neutralization titer of ⩾1:400 against human immunodeficiency virus (HIV)-HCV pseudotype, and 10 of 36 serum samples tested had a neutralization titer of ⩾1:20 against cell culture-grown HCV genotype 1a. Neutralizing serum samples had increased affinity levels and displayed >2-fold higher specific activity levels to well-characterized epitopes on E1/E2, especially to the hypervariable region 1 of E2.
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spelling pubmed-29314142011-09-15 Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins Ray, Ranjit Meyer, Keith Banerjee, Arup Basu, Arnab Coates, Stephen Abrignani, Sergio Houghton, Michael Frey, Sharon E. Belshe, Robert B. J Infect Dis Major Articles and Brief Reports Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 were used with MF59 adjuvant as a candidate vaccine for a phase 1 safety and immunogenicity trial. Ten of 41 vaccinee serum samples displayed a neutralization titer of ⩾1:20 against vesicular stomatitis virus (VSV)-HCV pseudotype, 15 of 36 serum samples tested had a neutralization titer of ⩾1:400 against human immunodeficiency virus (HIV)-HCV pseudotype, and 10 of 36 serum samples tested had a neutralization titer of ⩾1:20 against cell culture-grown HCV genotype 1a. Neutralizing serum samples had increased affinity levels and displayed >2-fold higher specific activity levels to well-characterized epitopes on E1/E2, especially to the hypervariable region 1 of E2. University Chicago Press 2010-09-15 2010-09-15 /pmc/articles/PMC2931414/ /pubmed/20677942 http://dx.doi.org/10.1086/655902 Text en © 2010 by the Infectious Diseases Society of America. All rights reserved. This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.
spellingShingle Major Articles and Brief Reports
Ray, Ranjit
Meyer, Keith
Banerjee, Arup
Basu, Arnab
Coates, Stephen
Abrignani, Sergio
Houghton, Michael
Frey, Sharon E.
Belshe, Robert B.
Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins
title Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins
title_full Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins
title_fullStr Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins
title_full_unstemmed Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins
title_short Characterization of antibodies induced by vaccination with Hepatitis C virus envelope glycoproteins
title_sort characterization of antibodies induced by vaccination with hepatitis c virus envelope glycoproteins
topic Major Articles and Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2931414/
https://www.ncbi.nlm.nih.gov/pubmed/20677942
http://dx.doi.org/10.1086/655902
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