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Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa

The main requirement for metabolic flux analysis (MFA) is that the cells are in a pseudo-steady state, that there is no accumulation or depletion of intracellular metabolites. In the past, the applications of MFA were limited to the analysis of continuous cultures. This contribution introduces the c...

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Detalles Bibliográficos
Autores principales: Lequeux, Gaspard, Beauprez, Joeri, Maertens, Jo, Van Horen, Ellen, Soetaert, Wim, Vandamme, Erick, Vanrolleghem, Peter A.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2934775/
https://www.ncbi.nlm.nih.gov/pubmed/20827435
http://dx.doi.org/10.1155/2010/621645