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Dynamic Metabolic Flux Analysis Demonstrated on Cultures Where the Limiting Substrate Is Changed from Carbon to Nitrogen and Vice Versa
The main requirement for metabolic flux analysis (MFA) is that the cells are in a pseudo-steady state, that there is no accumulation or depletion of intracellular metabolites. In the past, the applications of MFA were limited to the analysis of continuous cultures. This contribution introduces the c...
Autores principales: | Lequeux, Gaspard, Beauprez, Joeri, Maertens, Jo, Van Horen, Ellen, Soetaert, Wim, Vandamme, Erick, Vanrolleghem, Peter A. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2934775/ https://www.ncbi.nlm.nih.gov/pubmed/20827435 http://dx.doi.org/10.1155/2010/621645 |
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