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Stability of the Neurotensin Receptor NTS1 Free in Detergent Solution and Immobilized to Affinity Resin

BACKGROUND: Purification of recombinant membrane receptors is commonly achieved by use of an affinity tag followed by an additional chromatography step if required. This second step may exploit specific receptor properties such as ligand binding. However, the effects of multiple purification steps o...

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Detalles Bibliográficos
Autores principales: White, Jim F., Grisshammer, Reinhard
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935352/
https://www.ncbi.nlm.nih.gov/pubmed/20830205
http://dx.doi.org/10.1371/journal.pone.0012579