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Stability of the Neurotensin Receptor NTS1 Free in Detergent Solution and Immobilized to Affinity Resin
BACKGROUND: Purification of recombinant membrane receptors is commonly achieved by use of an affinity tag followed by an additional chromatography step if required. This second step may exploit specific receptor properties such as ligand binding. However, the effects of multiple purification steps o...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2935352/ https://www.ncbi.nlm.nih.gov/pubmed/20830205 http://dx.doi.org/10.1371/journal.pone.0012579 |