Counter-selection recombineering of the baculovirus genome: a strategy for seamless modification of repeat-containing BACs

Recombineering is employed to modify large DNA clones such as fosmids, BACs and PACs. Subtle and seamless modifications can be achieved using counter-selection strategies in which a donor cassette carrying both positive and negative markers inserted in the target clone is replaced by the desired seq...

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Detalles Bibliográficos
Autores principales: Westenberg, Marcel, Soedling, Helen M., Mann, Derek A., Nicholson, Linda J., Dolphin, Colin T.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938205/
https://www.ncbi.nlm.nih.gov/pubmed/20621982
http://dx.doi.org/10.1093/nar/gkq596

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938205/
https://www.ncbi.nlm.nih.gov/pubmed/20621982
http://dx.doi.org/10.1093/nar/gkq596

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