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Yeast targets for mRNA methylation

N(6)-Methyladenosine (m(6)A) is a modified base present in the mRNA of all higher eukaryotes and in Saccharomyces cerevisiae, where there is an increase in m(6)A levels during sporulation. The methyltransferase, Ime4, is responsible for this modification and has a role in the initiation of meiosis....

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Detalles Bibliográficos
Autores principales: Bodi, Zsuzsanna, Button, James D., Grierson, Donald, Fray, Rupert G.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938207/
https://www.ncbi.nlm.nih.gov/pubmed/20421205
http://dx.doi.org/10.1093/nar/gkq266
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author Bodi, Zsuzsanna
Button, James D.
Grierson, Donald
Fray, Rupert G.
author_facet Bodi, Zsuzsanna
Button, James D.
Grierson, Donald
Fray, Rupert G.
author_sort Bodi, Zsuzsanna
collection PubMed
description N(6)-Methyladenosine (m(6)A) is a modified base present in the mRNA of all higher eukaryotes and in Saccharomyces cerevisiae, where there is an increase in m(6)A levels during sporulation. The methyltransferase, Ime4, is responsible for this modification and has a role in the initiation of meiosis. However, neither the function, nor the extent of distribution of this nucleotide modification is established. We demonstrate that in S. cerevisiae, substantial levels of internal adenosine methylation are present in the GpA context in mRNA from sporulating cells, which is consistent with the preferred methylation consensus of higher eukaryotes. Based upon our quantification data, every second transcript could contain one m(6)A during meiosis. As methylation is distributed across all mRNA size ranges, it is likely that m(6)A is not limited to a small population of messages. We developed a new antibody based method for identifying m(6)A containing messages, and using this method the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself, were identified as being methylated. The position of m(6)A in IME2 was narrowed down to a region in the 3′-end. Methylation of these and other targets suggests mechanisms by which IME4 could control developmental choices leading to meiosis.
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spelling pubmed-29382072010-09-13 Yeast targets for mRNA methylation Bodi, Zsuzsanna Button, James D. Grierson, Donald Fray, Rupert G. Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics N(6)-Methyladenosine (m(6)A) is a modified base present in the mRNA of all higher eukaryotes and in Saccharomyces cerevisiae, where there is an increase in m(6)A levels during sporulation. The methyltransferase, Ime4, is responsible for this modification and has a role in the initiation of meiosis. However, neither the function, nor the extent of distribution of this nucleotide modification is established. We demonstrate that in S. cerevisiae, substantial levels of internal adenosine methylation are present in the GpA context in mRNA from sporulating cells, which is consistent with the preferred methylation consensus of higher eukaryotes. Based upon our quantification data, every second transcript could contain one m(6)A during meiosis. As methylation is distributed across all mRNA size ranges, it is likely that m(6)A is not limited to a small population of messages. We developed a new antibody based method for identifying m(6)A containing messages, and using this method the transcripts of three key, early regulators of meiosis, IME1, IME2 and IME4 itself, were identified as being methylated. The position of m(6)A in IME2 was narrowed down to a region in the 3′-end. Methylation of these and other targets suggests mechanisms by which IME4 could control developmental choices leading to meiosis. Oxford University Press 2010-09 2010-04-26 /pmc/articles/PMC2938207/ /pubmed/20421205 http://dx.doi.org/10.1093/nar/gkq266 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Bodi, Zsuzsanna
Button, James D.
Grierson, Donald
Fray, Rupert G.
Yeast targets for mRNA methylation
title Yeast targets for mRNA methylation
title_full Yeast targets for mRNA methylation
title_fullStr Yeast targets for mRNA methylation
title_full_unstemmed Yeast targets for mRNA methylation
title_short Yeast targets for mRNA methylation
title_sort yeast targets for mrna methylation
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938207/
https://www.ncbi.nlm.nih.gov/pubmed/20421205
http://dx.doi.org/10.1093/nar/gkq266
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