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In situ reverse transcription: the magic of strength and anonymity

In this study, we describe an approach that enables a highly specific, effective and fast detection of polyadenylated RNA sequences in situ at the light and electron microscopy levels. The method developed is based on the incorporation of 5-bromo-2′-deoxyuridine into the growing cDNA strand by means...

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Detalles Bibliográficos
Autores principales: Ligasová, Anna, Koberna, Karel
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938209/
https://www.ncbi.nlm.nih.gov/pubmed/20627869
http://dx.doi.org/10.1093/nar/gkq619