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In situ reverse transcription: the magic of strength and anonymity
In this study, we describe an approach that enables a highly specific, effective and fast detection of polyadenylated RNA sequences in situ at the light and electron microscopy levels. The method developed is based on the incorporation of 5-bromo-2′-deoxyuridine into the growing cDNA strand by means...
Autores principales: | Ligasová, Anna, Koberna, Karel |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938209/ https://www.ncbi.nlm.nih.gov/pubmed/20627869 http://dx.doi.org/10.1093/nar/gkq619 |
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