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Relative quantitation of transfer RNAs using liquid chromatography mass spectrometry and signature digestion products

Transfer ribonucleic acids (tRNAs) are challenging to identify and quantify from unseparated mixtures. Our lab previously developed the signature digestion approach for identifying tRNAs without specific separation. Here we describe the combination of relative quantification via enzyme-mediated isot...

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Detalles Bibliográficos
Autores principales: Castleberry, Colette M., Limbach, Patrick A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938229/
https://www.ncbi.nlm.nih.gov/pubmed/20587503
http://dx.doi.org/10.1093/nar/gkq578