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Relative quantitation of transfer RNAs using liquid chromatography mass spectrometry and signature digestion products
Transfer ribonucleic acids (tRNAs) are challenging to identify and quantify from unseparated mixtures. Our lab previously developed the signature digestion approach for identifying tRNAs without specific separation. Here we describe the combination of relative quantification via enzyme-mediated isot...
Autores principales: | Castleberry, Colette M., Limbach, Patrick A. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938229/ https://www.ncbi.nlm.nih.gov/pubmed/20587503 http://dx.doi.org/10.1093/nar/gkq578 |
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