A Proteomic Screen for Nucleolar SUMO Targets Shows SUMOylation Modulates the Function of Nop5/Nop58

Posttranslational SUMO modification is an important mechanism of regulating protein function, especially in the cell nucleus. The nucleolus is the subnuclear organelle responsible for rRNA synthesis, processing, and assembly of the large and small ribosome subunits. Here, we have used SILAC-based qu...

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Autores principales: Westman, Belinda J., Verheggen, Céline, Hutten, Saskia, Lam, Yun Wah, Bertrand, Edouard, Lamond, Angus I.
Formato: Texto
Lenguaje:English
Publicado: Cell Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938476/
https://www.ncbi.nlm.nih.gov/pubmed/20797632
http://dx.doi.org/10.1016/j.molcel.2010.07.025
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author Westman, Belinda J.
Verheggen, Céline
Hutten, Saskia
Lam, Yun Wah
Bertrand, Edouard
Lamond, Angus I.
author_facet Westman, Belinda J.
Verheggen, Céline
Hutten, Saskia
Lam, Yun Wah
Bertrand, Edouard
Lamond, Angus I.
author_sort Westman, Belinda J.
collection PubMed
description Posttranslational SUMO modification is an important mechanism of regulating protein function, especially in the cell nucleus. The nucleolus is the subnuclear organelle responsible for rRNA synthesis, processing, and assembly of the large and small ribosome subunits. Here, we have used SILAC-based quantitative proteomics to identify nucleolar SUMOylated proteins. This reveals a role for SUMOylation in the biogenesis and/or function of small nucleolar ribonucleoprotein complexes (snoRNPs) via the targeting of Nhp2 and Nop58. Using combined in vitro and in vivo approaches, both Nhp2 and Nop58 (also known as Nop5) are shown to be substrates for SUMOylation. Mutational analyses revealed the sites of modification on Nhp2 as K5, and on Nop58 as K467 and K497. Unlike Nop58 and Nhp2, the closely related Nop56 and 15.5K proteins appear not to be SUMO targets. SUMOylation is essential for high-affinity Nop58 binding to snoRNAs. This study provides direct evidence linking SUMO modification with snoRNP function.
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spelling pubmed-29384762010-10-13 A Proteomic Screen for Nucleolar SUMO Targets Shows SUMOylation Modulates the Function of Nop5/Nop58 Westman, Belinda J. Verheggen, Céline Hutten, Saskia Lam, Yun Wah Bertrand, Edouard Lamond, Angus I. Mol Cell Article Posttranslational SUMO modification is an important mechanism of regulating protein function, especially in the cell nucleus. The nucleolus is the subnuclear organelle responsible for rRNA synthesis, processing, and assembly of the large and small ribosome subunits. Here, we have used SILAC-based quantitative proteomics to identify nucleolar SUMOylated proteins. This reveals a role for SUMOylation in the biogenesis and/or function of small nucleolar ribonucleoprotein complexes (snoRNPs) via the targeting of Nhp2 and Nop58. Using combined in vitro and in vivo approaches, both Nhp2 and Nop58 (also known as Nop5) are shown to be substrates for SUMOylation. Mutational analyses revealed the sites of modification on Nhp2 as K5, and on Nop58 as K467 and K497. Unlike Nop58 and Nhp2, the closely related Nop56 and 15.5K proteins appear not to be SUMO targets. SUMOylation is essential for high-affinity Nop58 binding to snoRNAs. This study provides direct evidence linking SUMO modification with snoRNP function. Cell Press 2010-08-27 /pmc/articles/PMC2938476/ /pubmed/20797632 http://dx.doi.org/10.1016/j.molcel.2010.07.025 Text en © 2010 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Westman, Belinda J.
Verheggen, Céline
Hutten, Saskia
Lam, Yun Wah
Bertrand, Edouard
Lamond, Angus I.
A Proteomic Screen for Nucleolar SUMO Targets Shows SUMOylation Modulates the Function of Nop5/Nop58
title A Proteomic Screen for Nucleolar SUMO Targets Shows SUMOylation Modulates the Function of Nop5/Nop58
title_full A Proteomic Screen for Nucleolar SUMO Targets Shows SUMOylation Modulates the Function of Nop5/Nop58
title_fullStr A Proteomic Screen for Nucleolar SUMO Targets Shows SUMOylation Modulates the Function of Nop5/Nop58
title_full_unstemmed A Proteomic Screen for Nucleolar SUMO Targets Shows SUMOylation Modulates the Function of Nop5/Nop58
title_short A Proteomic Screen for Nucleolar SUMO Targets Shows SUMOylation Modulates the Function of Nop5/Nop58
title_sort proteomic screen for nucleolar sumo targets shows sumoylation modulates the function of nop5/nop58
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938476/
https://www.ncbi.nlm.nih.gov/pubmed/20797632
http://dx.doi.org/10.1016/j.molcel.2010.07.025
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