High-throughput production of human proteins for crystallization: The SGC experience

Producing purified human proteins with high yield and purity remains a considerable challenge. We describe the methods utilized in the Structural Genomics Consortium (SGC) in Oxford, resulting in successful purification of 48% of human proteins attempted; of those, the structures of ∼40% were solved...

Descripción completa

Detalles Bibliográficos
Autores principales: Savitsky, Pavel, Bray, James, Cooper, Christopher D.O., Marsden, Brian D., Mahajan, Pravin, Burgess-Brown, Nicola A., Gileadi, Opher
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938586/
https://www.ncbi.nlm.nih.gov/pubmed/20541610
http://dx.doi.org/10.1016/j.jsb.2010.06.008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938586/
https://www.ncbi.nlm.nih.gov/pubmed/20541610
http://dx.doi.org/10.1016/j.jsb.2010.06.008

Ejemplares similares