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Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage
Previously, we reported that Galactoxylomannan (GalXM) activates the extrinsic and intrinsic apoptotic pathways through an interaction with the glycoreceptors on T cells. In this study we establish the role of the glycoreceptor CD45 in GalXM-induced T cell apoptosis, using CD45(+/+) and CD45(−/−) ce...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939064/ https://www.ncbi.nlm.nih.gov/pubmed/20856869 http://dx.doi.org/10.1371/journal.pone.0012720 |
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author | Pericolini, Eva Gabrielli, Elena Bistoni, Giovanni Cenci, Elio Perito, Stefano Chow, Siu-Kei Riuzzi, Francesca Donato, Rosario Casadevall, Arturo Vecchiarelli, Anna |
author_facet | Pericolini, Eva Gabrielli, Elena Bistoni, Giovanni Cenci, Elio Perito, Stefano Chow, Siu-Kei Riuzzi, Francesca Donato, Rosario Casadevall, Arturo Vecchiarelli, Anna |
author_sort | Pericolini, Eva |
collection | PubMed |
description | Previously, we reported that Galactoxylomannan (GalXM) activates the extrinsic and intrinsic apoptotic pathways through an interaction with the glycoreceptors on T cells. In this study we establish the role of the glycoreceptor CD45 in GalXM-induced T cell apoptosis, using CD45(+/+) and CD45(−/−) cell lines, derived from BW5147 murine T cell lymphoma. Our results show that whereas CD45 expression is not required for GalXM association by the cells, it is essential for apoptosis induction. In CD45(+/+) cells, CD45 triggering by GalXM reduces the activation of Lck, ZAP70 and Erk1/2. Conversely, in CD45(−/−) cells, Lck was hyperphosphorylated and did not show any modulation after GalXM stimulation. On the whole, our findings provide evidence that the negative regulation of Lck activation occurs via CD45 engagement. This appears to be related to the capacity of GalXM to antagonize T cell activation and induce T cell death. Overall this mechanism may be responsible for the immune paralysis that follows GalXM administration and could explain the powerful immunosuppression that accompanies cryptococcosis. |
format | Text |
id | pubmed-2939064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29390642010-09-20 Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage Pericolini, Eva Gabrielli, Elena Bistoni, Giovanni Cenci, Elio Perito, Stefano Chow, Siu-Kei Riuzzi, Francesca Donato, Rosario Casadevall, Arturo Vecchiarelli, Anna PLoS One Research Article Previously, we reported that Galactoxylomannan (GalXM) activates the extrinsic and intrinsic apoptotic pathways through an interaction with the glycoreceptors on T cells. In this study we establish the role of the glycoreceptor CD45 in GalXM-induced T cell apoptosis, using CD45(+/+) and CD45(−/−) cell lines, derived from BW5147 murine T cell lymphoma. Our results show that whereas CD45 expression is not required for GalXM association by the cells, it is essential for apoptosis induction. In CD45(+/+) cells, CD45 triggering by GalXM reduces the activation of Lck, ZAP70 and Erk1/2. Conversely, in CD45(−/−) cells, Lck was hyperphosphorylated and did not show any modulation after GalXM stimulation. On the whole, our findings provide evidence that the negative regulation of Lck activation occurs via CD45 engagement. This appears to be related to the capacity of GalXM to antagonize T cell activation and induce T cell death. Overall this mechanism may be responsible for the immune paralysis that follows GalXM administration and could explain the powerful immunosuppression that accompanies cryptococcosis. Public Library of Science 2010-09-14 /pmc/articles/PMC2939064/ /pubmed/20856869 http://dx.doi.org/10.1371/journal.pone.0012720 Text en Pericolini et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pericolini, Eva Gabrielli, Elena Bistoni, Giovanni Cenci, Elio Perito, Stefano Chow, Siu-Kei Riuzzi, Francesca Donato, Rosario Casadevall, Arturo Vecchiarelli, Anna Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage |
title | Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage |
title_full | Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage |
title_fullStr | Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage |
title_full_unstemmed | Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage |
title_short | Role of CD45 Signaling Pathway in Galactoxylomannan-Induced T Cell Damage |
title_sort | role of cd45 signaling pathway in galactoxylomannan-induced t cell damage |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939064/ https://www.ncbi.nlm.nih.gov/pubmed/20856869 http://dx.doi.org/10.1371/journal.pone.0012720 |
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