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Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplification

A conserved nucleic acid fragment of the nucleocapsid gene of Swine Transmissible Gastroenteritis Coronavirus (TGEV) was chosen as the target, six special primers were designed successfully. Loop-mediated isothermal amplification (LAMP) was developed to detect the TGEV by incubation at 60°C for 1 h...

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Detalles Bibliográficos
Autores principales: Chen, Qin, Li, Jian, Fang, Xue-En, Xiong, Wei
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2939561/
https://www.ncbi.nlm.nih.gov/pubmed/20799985
http://dx.doi.org/10.1186/1743-422X-7-206