Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins

BACKGROUND: Overexpression of recombinant proteins usually triggers the induction of heat shock proteins that regulate aggregation and solubility of the overexpressed protein. The two-dimensional gel electrophoresis (2-DE)-mass spectrometry approach was used to profile the proteome of Escherichia co...

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Detalles Bibliográficos
Autores principales: Cheng, Chung-Hsien, Lee, Wen-Chien
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2940792/
https://www.ncbi.nlm.nih.gov/pubmed/20799977
http://dx.doi.org/10.1186/1475-2859-9-63

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2940792/
https://www.ncbi.nlm.nih.gov/pubmed/20799977
http://dx.doi.org/10.1186/1475-2859-9-63

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