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Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning
Targeted mutagenesis is one of the major tools for determining the function of a given gene and its involvement in bacterial pathogenesis. In mycobacteria, gene deletion is often accomplished by using allelic exchange techniques that commonly utilise a suicide delivery vector. We have adapted a wide...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Biomedical
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2941038/ https://www.ncbi.nlm.nih.gov/pubmed/20650290 http://dx.doi.org/10.1016/j.mimet.2010.07.014 |