Development of a Novel Fluorescence Assay Based on the Use of the Thrombin-Binding Aptamer for the Detection of O(6)-Alkylguanine-DNA Alkyltransferase Activity

Human O(6)-alkylguanine-DNA alkyltransferase (hAGT) is a DNA repair protein that reverses the effects of alkylating agents by removing DNA adducts from the O(6) position of guanine. Here, we developed a real-time fluorescence hAGT activity assay that is based on the detection of conformational chang...

Descripción completa

Detalles Bibliográficos
Autores principales: Tintoré, Maria, Aviñó, Anna, Ruiz, Federico M., Eritja, Ramón, Fàbrega, Carme
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946612/
https://www.ncbi.nlm.nih.gov/pubmed/20936180
http://dx.doi.org/10.4061/2010/632041

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946612/
https://www.ncbi.nlm.nih.gov/pubmed/20936180
http://dx.doi.org/10.4061/2010/632041

Ejemplares similares