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Development of a Novel Fluorescence Assay Based on the Use of the Thrombin-Binding Aptamer for the Detection of O(6)-Alkylguanine-DNA Alkyltransferase Activity

Human O(6)-alkylguanine-DNA alkyltransferase (hAGT) is a DNA repair protein that reverses the effects of alkylating agents by removing DNA adducts from the O(6) position of guanine. Here, we developed a real-time fluorescence hAGT activity assay that is based on the detection of conformational chang...

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Detalles Bibliográficos
Autores principales:	Tintoré, Maria, Aviñó, Anna, Ruiz, Federico M., Eritja, Ramón, Fàbrega, Carme
Formato:	Texto
Lenguaje:	English
Publicado:	SAGE-Hindawi Access to Research 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946612/ https://www.ncbi.nlm.nih.gov/pubmed/20936180 http://dx.doi.org/10.4061/2010/632041

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