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An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications

BACKGROUND: PCR amplification of minute quantities of degraded DNA for ancient DNA research, forensic analyses, wildlife studies and ultrasensitive diagnostics is often hampered by contamination problems. The extent of these problems is inversely related to DNA concentration and target fragment size...

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Detalles Bibliográficos
Autores principales: Champlot, Sophie, Berthelot, Camille, Pruvost, Mélanie, Bennett, E. Andrew, Grange, Thierry, Geigl, Eva-Maria
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2946917/
https://www.ncbi.nlm.nih.gov/pubmed/20927390
http://dx.doi.org/10.1371/journal.pone.0013042