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The use of reference gene selection programs to study the silvering transformation in a freshwater eel Anguilla australis: a cautionary tale

BACKGROUND: Quantitative real-time PCR (qPCR) has been the method of choice for the quantification of mRNA. Due to the various artifactual factors that may affect the accuracy of qPCR, internal reference genes are most often used to normalize qPCR data. Recently, many studies have employed computer...

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Detalles Bibliográficos
Autores principales: Setiawan, Alvin N, Lokman, P Mark
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949605/
https://www.ncbi.nlm.nih.gov/pubmed/20860839
http://dx.doi.org/10.1186/1471-2199-11-75