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The use of reference gene selection programs to study the silvering transformation in a freshwater eel Anguilla australis: a cautionary tale
BACKGROUND: Quantitative real-time PCR (qPCR) has been the method of choice for the quantification of mRNA. Due to the various artifactual factors that may affect the accuracy of qPCR, internal reference genes are most often used to normalize qPCR data. Recently, many studies have employed computer...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949605/ https://www.ncbi.nlm.nih.gov/pubmed/20860839 http://dx.doi.org/10.1186/1471-2199-11-75 |