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Slingshot: a PiggyBac based transposon system for tamoxifen-inducible ‘self-inactivating’ insertional mutagenesis

We have developed a self-inactivating PiggyBac transposon system for tamoxifen inducible insertional mutagenesis from a stably integrated chromosomal donor. This system, which we have named ‘Slingshot’, utilizes a transposon carrying elements for both gain- and loss-of-function screens in vitro. We...

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Detalles Bibliográficos
Autores principales: Kong, Jun, Wang, Feng, Brenton, James D., Adams, David J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2952874/
https://www.ncbi.nlm.nih.gov/pubmed/20688953
http://dx.doi.org/10.1093/nar/gkq658