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Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage

We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evalua...

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Detalles Bibliográficos
Autores principales: Rudi, Knut, Hagen, Irina, Johnsrud, Bente Carina, Skjefstad, Guro, Tryland, Ingun
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954551/
https://www.ncbi.nlm.nih.gov/pubmed/20948930
http://dx.doi.org/10.3390/ijerph7093376